Toluidine blue test for sperm DNA integrity and elaboration of image cytometry algorithm
نویسندگان
چکیده
منابع مشابه
Toluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays.
BACKGROUND Sperm DNA integrity (SDI) is an important factor in the prognosis of male fertility. Here we compare the toluidine blue (TB) image cytometry test, recently proposed by us for SDI assessment, with two other tests-the sperm chromatin structure assay (SCSA) and the terminal nick-end labelling (TUNEL) assay. METHODS Sperm samples from 35 men were evaluated for standard sperm parameters...
متن کاملThe utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis
OBJECTIVE The aim of the present study was to examine the relationship among male age, strict morphology, and sperm chromatin structure and condensation. METHODS Sperm samples from a total of 100 men underwent semen analysis, and sperm chromatin structure and condensation were assessed with toluidine blue (TB) and aniline blue (AB) tests. RESULTS Prevalence of strict morphology of less than...
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Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...
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Sperm genomic integrity and ultrastructural features of ejaculated spermatozoa contributing to the assessment of gamete fertility potential in patients with asthenozoospermia are discussed. The proportion of TUNEL-positive cells was significantly higher in the semen of patients with low sperm motility (n=40; p<0.01) as compared to men with normal sperm motility (n=54). Sperm DNA fragmentation n...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2003
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.a.10015